대한구강악안면방사선학회

Korean Academy of Oral and Maxillofacial Radiology

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방사선조사가 MC3T3-E1 조골세포주의 type I collagen과 alkaline phosphatase mRNA 발현에 미치는 영향
저자 : 최선영, 고광준
2003년|33권 1호|Page : 51-57|조회수 354
Full Text : 2003-18.pdf (201.8K)
Korean J Oral Maxillofac Radiol. 2003 Mar;33(1):51-57. Korean.

The effects of irradiation on the mRNA expression of type I collagen and alkaline phosphatase in the MC3T3-E1 osteoblastic cell line.

Choi SY, Koh KJ.

Department of Oral & Maxillofacial Radiology, School of Dentistry, Chonbuk National University, Korea. radkoh@moak.chonbuk.ac.kr
nstitute of Oral Bio Science, Chonbuk National University, Korea.

PURPOSE: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-E1 osteoblastic cell line, particularly an the expression of type I collagen and alkaline phosphatase mRNA.
MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.5, 1, 2, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. The specimens were then harvested and RNA extraction was carried out at 1 and 3 days after irradiation. The extracted RNA strands were reverse-transcribed and the resulting cDNA fragments were amplified by PCR.
RESULTS: The irradiated cells demonstrated a dose-dependent increase in type I collagen mRNA expression relative to the control group, with a maximum level of type I collagen mRNA expression occurring at 8 Gy. The degree of type I collagen mRNA expression increased significantly at 1 day after irradiation, but little differences were found between the control group and at the 3rd day. The amount of alkaline phosphatase mRNA expression increased significantly at 1 and 3 days after irradiation in the 1 Gy exposed group compared with the control group.
CONCLUSION: The amount of type I collagen and alkaline phosphatase mRNA expression increased significantly 1 day after irradiation when compared with the control group.

회비납부계좌 : 신한은행 100-031-466747 (예금주:대한영상치의학회) 입금 시
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