Korean Journal of Oral and Maxillofacial Radiology 2005; 35 : 191-8. English.

Effects of 2-deoxy-D-glucose and quercetin on cytokine secretion and gene expression of type I collagen during osteoblastic differentiation in irradiated MC3T3-E1 cells.

Song HU, Ahn HS, Lee SR, Koh KJ.

Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University, Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University Korea.

Purpose : To characterize the effects of 2-deoxy-D-glucose (2DG) and quercetin (QCT) on cytokine secretion of IL-6, TGF-β and gene expression of Col I in irradiated MC3T3-E1 cells Materials and Methods : The MC3T3-E1 cells were cultured in an α-MEM supplemented with 5 mM 2DG or 10 mM QCT and then the cells were incubated 12h before irradiation with 2, 4, 6, and 8 Gy X-ray using a linear accelerator delivered at a dose rate of 1.5 Gy/min. Level of IL-6 and TGF-β was determined by ELISA. Also expression of Col I was examined by RT-PCR.
Results : In accordance with the radiation dose, the amount of TGF-β was not different in RA±QCT, but it showed a peak value in control and RA±2DG at 4Gy on the 3rd day. However, all groups showed a decreasing tendency dose-dependently in RA±QCT on the 7th day (p⁄0.01). In accordance with the radiation dose, the amount of IL-6 increased dose-dependently in all groups on the 3rd day. On the 7th and 21st day, all groups showed peak values at 4Gy. RA±QCT showed a slightly increased amount of IL-6 at 2 Gy, but it showed a slightly decreased amount at 4, 6, and 8 Gy. In accordance with the period of culture after irradiation, the expression of Col I increased dosedependently in RA±QCT.
Conclusion : The result showed that QCT acted as radiosensitizer in the secretion of TGF-β and gene expression of Col I during differentiation in irradiated MC3T3-E1 cells at the cellular level. (Korean J Oral Maxillofac Radiol 2005; 35 : 191-8)
KEY WORDS : 2-deoxy-D-glucose; Quercetin; Radiation; MC3T3-E1 Cells